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The BN-gels were scanned and the optical density along each lane was quantified (Quantity one, Bio-Rad, USA) to determine the relative mobility.
Total intensities were quantified (Quantity One, Bio-Rad).
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The relative intensity of bands was quantified using Quantity One analysis system (Quantity One, Hercules, CA, USA).
The relative quantities of each protein band, normalized to control cells, were quantified using Quantity One software (version 4.6.5, Bio-Rad Inc, Hercules, CA, USA).
We quantified the quantity of cleaved heteroduplexes and we expressed our results as percentages of cleaved heteroduplexes relative to the total quantity of DNA.
The bands were quantified by Quantity One software.
The intensity of the bands was quantified using Quantity One software and plotted against the time after cycloheximide treatment.
Bands were quantified with Quantity One® software.
Signals were quantified with Quantity One software (Bio-Rad).
Protein levels were quantified with Quantity One Software (Bio-Rad) and normalized with actin level.
The intensity of the product was quantified using Quantity One, Bio-Rad.
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