Cells were treated with 1 µmol/L Ponatinib for 8 h, the migrating cells were stained with crystal violet (left panel) and quantified (right panel).
Cells were treated with 1 µmol/L Ponatinib, the wound width was photographed after scratching (left panel) and quantified (right panel).
(H) LM2 cells were treated with DMSO or 1 µmol/L Ponatinib for 24 h, Western blot analysis of the indicated protein level and phosphorylation level (left panel), related p-ERK level were quantified (right panel).
The immunoblot results were quantified (left panel) using α-tubulin as a loading control on a representative gel (right panel).
The results were quantified (left panel) using α-tubulin as a loading control on a representative gel (right panel).
In the later this border becomes wiggly and inter-digitized, a feature quantified in panel G.
For method validation purposes, we quantified a panel of 16 genes via real time quantitative reverse transcription PCR (qRT-PCR) using the same MDM-extracted RNA samples that were used for the RNA-seq and microarray platforms as described above.
(E ) Gel shift assays with the 234-nt RRE and full-length Rev, visualized using SYBR Green II staining, show reduced accumulation of dimer species and a modest loss of binding affinity with the Gln51Ala mutant, quantified in panel (F ).
Signals in panels (a c) were visualised with Chemi Doc XRS imager, and quantified (in panels (a,b)) using Quantity One® software.
Finally, we performed Western blots for Survivin, T-Antigen and IGF-IR in (Panel C) and quantified their levels (Panel D) in the neurospheres used for cell cycle analysis and detection of apoptosis.
However, we were surprised to observe sizeable Okazaki fragments (autoradiograph illustrated in Figure 1D left panel and quantified on the right panel).
