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Number of colonies was quantified manually.
Single- or double-labeled neurons were quantified manually or by using NIH Image J Software.
48 h after plating cells, images were taken and total number of cells per field was quantified manually on ImageJ.
For morphometric analysis of mitochondrial cristae, performed in a blind fashion on at least five mitochondria/cell from six randomly selected cells (n = 3 independent experiments), maximal cristae width was measured using the ImageJ Multimeasure plug-in3; the number of horizontal cristae and cristae junctions were quantified manually.
GNPs were subsequently quantified manually with a hemacytometer and snap frozen in liquid nitrogen.
Behavior was recorded with a video camera and the time spent immobile (making only those movements necessary to keep the snout above the water), swimming, climbing or diving was quantified manually using a computer program (The Observer 5.0.25, Noldus IT, 2003).
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The amount of wing beats during flapping was relatively simple to quantify manually from video, as the birds performed it at a slow rate.
Applying our analytic software, we successfully quantified the 9 to 17 H3 peptides (KSTGGKAPR) containing serine 10 phosphorylation that otherwise would be difficult to quantify manually due to their low abundance.
Foci from individual nuclei were quantified by manually scanning through compiled Z stacks.
Golgi recovery was quantified by manually counting the percent of cells displaying a perinuclear location of GM130.
However, cell migration using this method was quantified by manually counting cells (which is a tedious and time consuming process).
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