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The ELISPOT responses one week after vaccination reached or exceeded the maximal readily quantified level for the majority (60%) of subjects.
In addition to calculating comorbidity with our instrument, for each respondent we quantified level of comorbidity using two other validated comorbidity measurement tools.
In addition, we quantified level IV ecoregions (USEPA 2007) occurred within the boundaries of each subwatershed; we used these ecoregions as proxies for overall habitat characteristics as they identify areas of similar climate and landscape characteristics (Omernik 1987; USEPA 2007).
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We quantified levels of 2082 protein spots and identified 272 (170 unique UniProt accessions) by mass spectrometry.
As a control for the specificity of shdBili we also quantified levels of armadillo mRNA and found no change (Fig. S2A).
We quantified levels of total phenolics as above.
As a control, we also quantified levels of Lamin mRNA in these cells (Fig. 6E).
We additionally quantified levels of the principal protein scavenger of mitochondrial superoxide, MnSOD (SOD2).
Figure 4 shows quantified levels of HIF-1 α immunoexpression in renal tissues.
Figure 6 shows quantified levels of TGF- β1 immunoexpression in renal tissues.
Serum procalcitonin levels greater than 0.05 ng/ml were quantified; levels <0.05 ng/ml were interpreted as negative.
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