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mRNA and protein levels of IFNγ, TNFα and IL-2 were quantified in parallel.
Cell death in response to HAMLET was quantified in parallel in Jurkat leukemia cells and S. pneumoniae (Fig. 1A).
Comparison of transcriptome reads of the same samples quantified in parallel from AS and SMS platforms reveals a distinct bias in AS results towards a slight overrepresentation of highly expressed genes as compared to SMS, as shown in Figure 1A.
The RPP30 cellular gene was quantified in parallel to normalize sample input.
β-actin mRNA was quantified in parallel as the reference control.
Glycolysis was quantified in parallel on the same samples by measuring the ECAR as previously described.
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Areas stained positive for α-SMA were quantified in a parallel fashion.
PMN adhesion to HUVEC was quantified in a parallel plate flow chamber with a laminar flow profile (Reynolds number <1, Figure 1) at 37°C as previously reported [ 18].
Free F5 was quantified in 5 parallels as described for quantification of RT-PCR products.
Cell numbers of non transfected cells have been quantified once in parallel by thymidine incorporation assay.
These events were quantified in live cell recordings, in parallel with automatic cell quantification based on Hoechst stain of macrophage nuclei (Fig. 2B, Video S2).
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