Sentence examples for quantified in duplicate from inspiring English sources

A specific standard curve was performed in parallel for each gene, and each sample was quantified in duplicate.

Plasma PI3, SLPI, and HNE levels were quantified in duplicate using Human pre-ELAFIN/SKALP (Cat. No. HK318), Human SLPI (Cat No. HK316), and Human Elastase (Cat No. HK319) ELISA Test Kit from Cell Sciences (Canton, MA), according to the manufacturer's recommended protocol.

Each sample was quantified in duplicate.

VEGF165 in the supernatants was quantified in duplicate by ELISA Kits (Lifekey Corp).

The samples were amplified by PCR and methylation was quantified in duplicate.

TNF-α mRNA were quantified in duplicate by SYBR green two-step, real-time RT-PCR.

cDNA of each brain was quantified in duplicates.

Cells suspended with PBS (with Ca++ and Mg++) were seeded at 2.5×105 cells/well in 100 µl in the coated wells for 30 min. Non-adherent cells were removed by washing, and adherent cells were stained by 0.05% crystal violet and quantified in duplicates by light absorbance (562 nm).

All target and reference mRNAs were quantified in duplicates for all biological replicates, respectively.

Serum levels of irisin, leptin, adiponectin and resistin were quantified in duplicates by ELISA.

TNF-α and GM-CSF were quantified in duplicates using DuoSet ELISA Development System Kits (R&D Systems Europe, UK) according to the manufacturer's instructions.