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Next, 50-μm tumor sections were immunostained for VE-cadherin, imaged by confocal microscopy, and quantified for volume of vascular leakage in relation to the total vessel volume using Imaris image analysis software (Bitplane) as previously detailed (8, 31).
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Five variables were quantified for muscle volume; total quadriceps volume (VI + VM + RF + VL) along with each muscle contribution (individual muscle volume divided by the total quadriceps volume expressed in %).
This allows a maximum safe loading of construction materials to be quantified for a given volume of enclosure.
FASN abundance was quantified for each sample and a volume of cytosolic lysate representing 5 μg of FASN per sample was analyzed for FASN activity as previously described [ 4, 21].
Two important geometric parameters, volume and surface area, were quantified for left and right talus bones.
Here we found that EC and PC, two of the major lipid classes recently quantified for BrM lipoproteins [39], together accounted for at least 40% of druse volume.
Synapse flat areas were also traced and quantified for five additional dendrites (D001, D002, D007, D009, and D010) in the apical dendrite volume.
In general, plasma sample volumes were not sufficient to facilitate the analysis of more than one steroid; therefore, E2 was quantified for females and T was quantified for males.
The dot intensities of apoptosis membrane arrays for semi-quantitative analysis were quantified by volume densitometry using BioRad Image Lab software.
Isoforms were named in reference to those shown in Fig. 2. The expression level of DJ-1 isoforms vs. total DJ-1 level for each group was measured and quantified as volume percentage (Fig. 4B).
For quantitative immunoblotting, known amounts of FtsZ or ClpX were quantified by volume analysis function of the QuantityOne software and standard curves were plotted.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com