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Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA was quantified for use as an internal control.
When interpreted by modelers, results of landscape-level behavioral experiments can be quantified for use in predictive models.
The relationship between BMI and dietary fat intake (% of daily calories due to fat) is shown in Figure 2. Physical activity was also quantified for use in the current study.
Genomic DNA was prepared for use in the various steps of assay design and optimization using the QIAamp DNA Mini Kit (QIAGEN, Valencia, California) using Protocol C then quantified for use in standard curve experiments using a NanoDrop ND-1000 or 8000 spectrophotometer (Nanodrop Technologies, Wilmington, Delaware).
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All amplified libraries (pre-capture preparation with NEBNext or ThruPLEX) were purified with Agencourt AMPure XP beads (cat. no A63881) and quantified for sequencing using 2100 Bioanalyzer High sensitivity kit (cat. no 5067-4626).
To determine if this effect was also observed with a more direct measure of proliferation duplicate cultures were quantified for proliferation using BrdU.
Test flavonoids in combination or alone were quantified for activities using serial broth half dilution method (Table 5).
Serum samples obtained from each patient were quantified for MCP-1 using enzyme-linked immunosorbent assay (ELISA).
Subsequently, the amount of free, membrane-bound, and internalized activity were quantified for each sample using a γ-counter.
Samples were quantified for protein concentration using the BioRad Protein Assay Kit (BioRad, Canada).
Images were quantified for green fluorescence using the image analysis software Image J (NIH).
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