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Results show the system's use as a pollutant detector, with benzene quantified down to 500 ppm using a PVC polymer coating.
Different Legionella species might be rapidly quantified down to 4×103 cells/m3, which fulfills the requirements for bioaerosol measurements in the environment and in the interior.
With PTR-MS, many VOCs can be quantified down to the 10 100 parts per trillion range with reliable results (Biasioli et al. 2011; de Gouw et al. 2003; Lindinger et al. 1998).
Utilizing the proposed facile colorimetric scheme, the target DNA can be quantified down to 4 pM with the dynamic response range of 5 orders of magnitude, indicating the substantially improved detection capability.
After protein precipitation of 100 μl plasma sample aliquots with perchloric acid, the analytes are directly injected, separated within 10 min and simultaneously quantified down to 20 ng/ml by an electrochemical detector equipped with a dual-electrode system operating in redox mode eliminating effectively potential endogenous and exogenous interferences.
The β-globin reference gene was quantified down to a limit of 1.5 pg of DNA/μl (approximately 5 cells) with intra- and interassay coefficients of variability ranging from 1.8%to21%1.8%to21%
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Western Blotting was used to determine the level of expression of CDC2 protein as measure to quantify down regulation of CDC2 expression along with use of flow cytometry to investigate effect of shRNAs on cell cycles and detection of apoptosis.
Therefore, we developed and validated a digital PCR method to detect and accurately quantify chimerism down to 0.01% recipient cells.
In order to look at metabolism in more detail, we employed a dose of ~100 nmol which allowed us to quantify metabolites down to 0.001 %ID/g.
For example, we were able to quantify methotrexate down to 5 nM in plasma (data not shown).
In the last 10 years proteomics technologies involving mass spectrometry have made it possible to quickly identify proteins and quantify adducts, down to the very amino acid site of modification, so scientists can more quickly screen potential biomarkers.
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