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The fluorescence intensity of each individual cell was quantified digitally as pixel number.
The amount of positive macrophage staining was quantified digitally with Image Pro Plus software version 5.0.
Band density was quantified digitally using the ChemiImager 4400, version 5.5 (Alpha Innotech, San Leandro, CA).
These data were quantified digitally and were confirmed via manual imaging of the TMA slides (Additional file 2: Figure S2B).
EROD activity was measured as intensity of the bladder fluorescence and was quantified digitally by IPLab software (Scanalytics Inc., Fairfax, VA).
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Removal was quantified by digitally photographing the biochar samples and visually estimating disappearance over time.
Error rates, false positives and false negatives along with true positives and true negatives are quantified by digitally comparing results with manually determined borders from a dermatologist.
Tumor cell escape and invasion into the mesoderm were then visualized with an epifluorescent microscope and quantified in digitally acquired images.
Error rates: false positives and false negatives along with true positives and true negatives are quantified by digitally comparing results with manually determined borders from a dermatologist as the ground truth.
The BH detected borders and interiors (e.g. combination of branches 3 and 4 of Fig. 2), overlaid images, and dermatologist-determined region are all illustrated, respectively, in Figure 4. Second, accuracy of our method is quantified by digitally comparing results with manually determined borders from a dermatologist.
Nitration of Mn-SOD was digitally quantified as aforementioned.
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