Surface loads were quantified by determining the point of zero charge (pHPCZ) of the adsorbents.
Primary hyperalgesia was quantified by determining mechanical (von Frey hairs) and thermal (Peltier thermode) pain thresholds.
The concentration of the attached bacteria was quantified by determining the viable colony forming unit (CFU/cm2) numbers.
Proliferative and apoptotic cells were quantified by determining the percentage of Ki-67-positive and TUNEL-positive cells, respectively.
Secondary hyperalgesia was quantified by determining the area in which normally unpleasant von Frey hairs evoked pain or tenderness.
The fracture toughness was quantified by determining the critical strain energy release rate (GIC) using the modified beam theory.
Xylanase activity was quantified by determining the amount of reducing sugar released from xylan derived from birchwood according to Bailey et al. (1992).
Blood vessel density in s.c. tumors was quantified by determining the ratio of the CD31-stained area to the total tumor area within the respective field of view.
Total RNA was isolated from frozen mycelia harvested at different fermentation times, using TRIZOL (Invitrogen) extraction, and was spectrophotometrically quantified by determining the absorbance ratio at OD260/280.
