Cells were plasma-treated as described and incubated for an additional 3 days with a medium change on day 2. Cell number was quantified on days 1 and 3 by counting trypsin-detached cells using a Cell Viability Assay (Guava EasyCyte Plus, Millipore, MA, USA).
Mice were loaded on 3 consecutive days and bone formation was quantified 16 days after initiation of loading.
O. crenata seed germination and attachment were observed and quantified 21 days after inoculation, while nodules were observed and counted 4 days later.
Pictures of all RNAi worms were taken (n = 12 each, except for B-1 where n = 10) on days 1, 4, 6, and 8 after decapitation and eye appearance was quantified for days 4, 6, 8.
To investigate how effectively iron was used in each of these groups, the stable iron isotopes Fe and Fe were administered orally on days 1 and 15, respectively, and incorporation of each stable iron isotope into erythrocytes was quantified 14 days after its administration.
Early apoptosis of MSCs in control, wo CPA, DMSO, Sucrose and DMSO + Sucrose cryopreserved samples was quantified 7 days after thawing with the Annexin-V-FITC Apoptosis Detection Kit (Sigma-Aldrich).
The brown lesions characteristic of F. solani infection, observed on the system Radical (Espinosa-Victoria et∼al. 2004), were quantified 30 days after inoculation with the pathogen, with the aid of a stereoscopic microscope, in 10 seedlings of each treatment.
Both groups of animals were subsequently challenged with wild-type C. neoformans strain H99 that does not produce IFN-γ, and the fungal burden was quantified on days 3, 7, and 14 post-inoculation.
HCV RNA was determined by RT-PCR using Cobas Amplicor HCV Monitor version 2.0 (Roche Diagnostics, Branchburg, NJ), and quantified on days 0, 1, 4, 7, 8, 15, 22, 29, at end of treatment, and 24 months after the completion of treatment.
Liver damage was quantified at days 3 and 7 PI by measuring serum levels of alanine transaminase (ALT) using commercially available Infinity™ ALT reagents (Thermo Scientific, Waltham, MA) according to manufacturer's instructions.
To test this hypothesis, wt bone marrow cells were directly injected into the thymic lobes of wt and ADAM8−/− recipient mice, and donor cells in the respective recipient thymi quantified 10 days later.
