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CRP was quantified by utilizing latex-enhanced nephelometry with a Behring Nephelometer Analyzer System.
Images were taken of six random optical fields (200×) on each filter and cell number was quantified by utilizing the Image-Pro Plus analysis software (Media Cybernetics).
C-Reactive protein (CRP) was quantified by utilizing latex-enhanced nephelometry with a Behring Nephelometer Analyzer System (Behring Diagnostics, Frankfurt, Germany).
The mitochondrial DNA content was quantified by utilizing a fluorometric assay where an increase in SYBr Green (Invitrogen Molecular Probes) fluorescence because of higher DNA binding was quantified as a measure of the amount of DNA content.
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EPCs were quantified by flow cytometry, utilizing the surface markers CD34, CD133, CD45; for the mature circulating endothelial cells (CECs, indices of endothelial injury), CD34, CD45, VEGFR2 were utilized.
Two micrograms of total RNA was then reverse-transcribed, and quantified by RT-qPCR utilizing the following primer sets and probe Sense: 5'-TCTTCACGCAGAAAGCGTCTA-3', Anti-Sense 5'-CGGTTCCGCAGACCACTATG-3', Taq-man FAM labeled probe 5'-/56-FAM/TGAGTGTCG/ZEN/TGCAGCCTCCAGGA/3IABκFQ/-3' 5'-/56-FAM/TGAGTGTCG/ZEN/TGCAGCCTCCAGGA/3IABκFQ/-3' 5'-/56-FAM/TGAGTGTCG/ZEN/TGCAGCCTCCAGGA/3IABκFQ/-3' 5'-/56-FAM/TGAGTGTCG/ZEN/TGCAGCCTCCAGGA/3IABκFQ/-3'
To evaluate the fibrosis in each muscle, the amount of intermyofibre connective tissue formed in selected muscles was quantified in situ by utilizing the specific binding of wheat-germ agglutinin (WGA) to N-acetylglucosamine and sialic acid residues in proteoglycans, polysaccharides and glycoproteins in the extracellular matrix (Dunn et al. 1982; Kostrominova 2011).
Furthermore, incremental electromagnetic field exposure in addition to the one acquired in subjects' working and living environment was quantified by the degree of utilizing a mobile phone and could not be shown to influence the risk of tinnitus (p = 0.5116).
From these results, a new assay method for quantifying lysozyme was established by utilizing the Morgan-Elson reaction based on the generation of product D (2-acetamide-2,3-dideoxy-glucopyranose), which serves as a chromophore, formed from Gal(GlcN 3D by lysozyme through a conjugated reaction involving β-N-acetylhexosaminidase.
In this study we extend these approaches to quantify repetitive element enrichment by utilizing all mapping reads in estimating read counts.
Thereby we were able to differentiate treated from untreated MCF-7 cells and to quantify the cell drug response by utilizing linear discriminant analysis models.
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