Sentence examples for quantified by two different from inspiring English sources

EN282 was detected and quantified by two different strategies: (1) its growth on ENSM and amplifying its DNA using conventional PCR, and (2) quantifying its DNA using real-time PCR.

CNAs levels in plasma from twenty-one acute P. vivax malaria patients from the Brazilian Amazon and 14 malaria non-exposed healthy donors were quantified by two different methodologies: amplification of the human telomerase reverse transcriptase (hTERT) genomic sequence by quantitative real time PCR (qPCR), and the fluorometric dsDNA quantification by Pico Green.

Aliquots of the inactivated virus stock solutions were lyophilized and redissolved, and the virus RNA was quantified by two different noncommercial real-time RT-PCR assays (2, 6 ).

Adherent cells are then quantified by three different fluorescence-based techniques including spectrofluorimetry, ELISA-type cytofluorimetry and fluorescence microscopy coupled to digital image quantification.

Comprehensive investigation of the plasmid RMs was performed for their sequence, purity, homogeneity and stability, and then the concentration was quantified by three different methods: ultraviolet spectrophotometer (UV), high resolution inductively coupled plasma mass spectrometry (HR-ICP-MS) and digital PCR.

Apoptosis was quantified by four different methods.

The extent of air trapping was quantified by four different methods: The percentage of lung voxels below -850HU in expiration (EXP−850) [ 20, 21 ].

Neuronal apoptosis was quantified by three different approaches: (i) cells were incubated with the DNA-binding fluorochrome Hoechst 33342 (10 μg/ml) for 10 minutes prior to fixation with 4% paraformaldehyde.

This sites were quantified by three different glycopeptides, i.e. DIEITN*ATIQSEEDSR (N* indicates that asparagine was detected with deamidation) with heavy/light ratio 0.85 by trypsin digestion, ITN*ATIQSEEDSR with heavy/light ratio 1.03 by trypsin & Glu-C digestion, and IIIHSKDIEITN*ATIQSEEDSRY with heavy/light ratio 1.12 by chymotrypsin digestion.

Since cervico-vaginal washing fluids are rather complex media, we quantified HMGB1 by two different methods, a commercial ELISA and a band shift assay that is less prone to interfering with masking proteins that might associate with HMGB1 in vivo [30].

The physical titer (viral particles/ml, VP/ml) was quantified by two methods that measure different constituents of the particles, i.e. the lentiviral capsid protein p24 or the viral genome as measured with quantitative Realtime PCR (QRTPCR).