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Alignment of Schlemm's canal endothelial cells in human eyes was quantified by scanning electron microscopy.
After 4 h incubation under the same conditions, the cleavage of MTT to formazan by metabolically active cells was quantified by scanning the plates at 540 nm using a Multiskan EX (Finland) apparatus.
Relevant bands were quantified by scanning densitometry.
Dark brown staining in nucleus represents phospho-ELK and was quantified by scanning slides using related software.
Bands were quantified by scanning the film with a Molecular Dynamics Densitometer and quantifying bands with Image Quant 5.2 software (Molecular Dynamics).
The amounts of the proteins in the samples were quantified by scanning profiles using the Scion imaging program (Scion, Frederick, MD).
The relative amount of transferred protein in a given sample was quantified by scanning Xrays films and by estimating the relative arbitrary density units using the ImageQuant software.
Protein levels were quantified by scanning densitometry of the autoradiography films using VersaDoc 3000 Gel Imaging System (BioRad, CA, USA) and normalized over (ratio) the housekeeping protein levels.
The results showed that the amounts of EGR-1 were higher in stimulated BeWo cells than in control cells (Fig. 4B); autoradiograms quantified by scanning densitometry revealed a relative enrichment of 1.47±0.23.
Bound complexes were detected and quantified by scanning densitometry.
Immunoblots were quantified by scanning densitometry and ImageJ software.
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