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Quantified analysis results were shown in Figure 9(b).
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To validate the microarray analysis results, expression of selected genes was quantified by RT-qPCR.
The upper right triangle quantifies the single-sided linkage results whereas the lower left triangle represents the number of two-sided linkage analysis results.
Analysis Results.
However, we were able to conduct a meta-analysis, quantifying the results of the studies.
Analysis result proves that tangential force can be used to quantify the effect of fretting.
Nevertheless, we quantified the results by creating a calibration curve of standard solutions and correcting for the spatial non-uniformities (see Methods for details on image analysis).
Optional: quantify the results using imaging analysis software.
In order to validate and quantify the results of PCR analysis we carried out Southern blot hybridisation.
Densitometric analysis was performed using Image Quant software (Molecular Dynamics, Sunnyvale, CA, USA) to quantify the results of Northern blot analysis (VEGF, NRP-1, and GAPDH mRNA expression) in the linear range of the film.
Relative standard curve analysis (SDS2.0 software; Applied Biosystems) was used to quantify products generated, and all quantified results were normalised to 18S, and subsequently to POU5F1, transcript levels.
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