Exact(12)
To eliminate noise and artifacts as good as possible, the exact masses given in Table 5 were determined with the standards, and the mass window for quantification was set to ±0.01 m z-1.
The absolute lower limit of quantification was set at the mean analyte area in the zero samples +10 times the standard deviation.
The temperature profile was 95°C for 10 min followed by 40 cycles of denaturation at 95°C for 15 s and annealing/extension at 60°C for 1 min. The cycle threshold for DNA quantification was set to 0.2 for all experiments in this study.
The lower limit of quantification was set at 1 : 50.
The lower limit of quantification was set to 1.0 ng ml−1.
The lower limit of quantification was set to 1.5 ng/mL.
Similar(48)
The limits of quantification were set to 4 ng/mL and 10 ng/g for plasma and tissues, respectively (coefficient of variation and overall bias less than 30%).
The area under the concentration-time curve (AUC) between 0 and 31 h (AUC0 31) was determined using the trapezoidal rule in which concentrations less than the lower limit of quantification were set at zero.
The abundance of the target relative to GAPDH was calculated as: Xn = (1 + EGAPDH Ct,gapdh/(1 + Etarget Ct,target, where Ct is the threshold cycle determined from the amplification curves, and the relative abundances from one quantification were set into relation with one another.
Thus, in accordance with published recommendations [56], the quantification limit was set at 50 copies per sample.
The quantification method was set to SILAC 2plex (Arg6, Lys6) and light/heavy ratios were reported.
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