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The matrix effect (ME%) from coexisting NP-matrix constituents on quantification was estimated by comparing the signal peak area of the target analytes in neat water-acetonitrile (1 1, v/v) solution with that of the same amount of the analyte spiked into a post prepared supernatant from 200 μL of the NP-containing blank sample (postprecipitation spike).
Transcript quantification was estimated from RNA-seq data using the RSEM software package.
Quantification was estimated by band densitometry on 2% agarose gels (Gene Tools software; Syngene, Cambridge, UK).
Transcript quantification was estimated from RNA-Seq data using the RSEM software package contained in the Trinity suite.
Crocetin esters quantification was estimated using the method based on the extinction coefficient and the related area calculated according to [ 26, 27].
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Despite the high constancy of the sensitivity of the well counter over time, the systematic and random errors committed on activity quantification were estimated at −12.89 [16.55] % (N = 34) for measurements based on the residual patient activity.
RNA purity (A260/A280 nm) and quantification were estimated using a Nanodrop 1000 spectrophotometer (Thermo Fisher Scientific Inc., Wilmington, DE, USA).
qPCR efficiencies were determined using linear regression analysis [ 39, 40] using LinRegPCR software and relative quantifications were estimated with the Pfaffl-method [ 41].
Quantification limit (LO Q was estimated from LOQ from the equation: {text{LOQ}} ; = ;{text{LOD}}; times ;3.
The average method limit of quantification (LOQ) was estimated at 0.77 ng/g for placenta samples and 1.2 ng/g for fetal liver samples based on 10 times the signal to noise ratio.
The compound relative quantification of MMS was estimated using a gas chromatograph Shimadzu GC-2010 with a SLB-5 ms non-polar capillary column 5%diphenyl/95%% dimethyl siloxane; 30 m × 0.25 mm × 0.25 μm) and a flame ionization detector.
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