The tissue was prepared and protein quantification was determined using the BCA protein assay.
Finally, the accuracy of the quantification was determined for the large uniform part of three different phantoms.
The limit of quantification was determined by identifying the next highest concentration on the standard curve with a signal-to-noise ratio of >10.
With regard to organochlorine pesticide analyses, the current practice for limits of detection and quantification was determined as part of the method development and validation.
The accuracy of the quantification was determined by comparing the measured activity with the known activity within the phantom measured at preparation.
Quantification was determined using multiple reactions monitoring model, and the operating parameters were optimized as follows: drying gas (N2) flow rate, 10.0 L/min; drying gas temperature, 350 °C; nebulizer, 45 psi; capillary, 3500 V; fragmentor voltage, 150 V; sheath gas temperature, 350 °C; sheath gas flow rate, 11 L/min.
Protein quantification was determined by BCA assay.
Absolute quantification was determined using a genomic DNA standard curve.
Protein quantification was determined by Bio-Rad Protein Assay reagent (Bio-Rad, Cat# 500 0006).
The single strand DNA profile and quantification was determined by running 1 µl of the samples on the Agilent Bioanalyzer 2100 using a RNA Pico 6000 chip.
Quantification was determined by counting the number of immunoreactive (ir) cells in each experiment divided by the total number of cells (DAPI-ir cells) in the same experiment.
