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Relative quantification was calculated by extrapolation of the standard curve and calculation of ratio levels compared with β-Actin.
The relative quantification was calculated according to the manufacturer's instructions (Applied Biosystems).
The numerical value of the error of quantification was calculated as the percentage difference of the object activity determined using each of the analyzed methods relative to the "true" activity as measured in the dose calibrator.
The relative error on quantification was calculated as the difference between the activity found with the considered calibration factor and the ground truth, divided by the ground truth value.
Relative quantification was calculated using the 2-ΔΔCt method.
Quantification was calculated using the comparative Ct method.
The relative quantification was calculated with the analysis software provided with the iCycler IQ5 (Biorad).
Relative quantification was calculated using the equation 2−ΔCT = CTGENE-CTGAPDH.
The protein density of the bands was quantified using Quantity One software v 4.2.6, and relative quantification was calculated with reference to the á-tubulin signal.
The correlation between numbers of Solexa reads and PCR quantification was calculated by Pearson's correlation analysis and the statistical significance was assigned by Student's t test.
Relative quantification was calculated by the ΔΔCT method, with expression of strain 923 at 2 hrs as the reference, as described [7].
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