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DNA concentration was determined through quantification using the BioPhotometer spectrophotometer (Eppendorf).
(D) The short isoform abundance quantification using the relative gray value for band signal in C by imageJ.
The notion has the benefit of unambiguous uncertainty quantification, using the well-established mathematical framework of probability and measure theory.
The target gene expression levels were determined by relative quantification using the comparative CT method, also known as 2-∆∆CT method (comparative 2 [delta][delta]Ct method).
Analyzing the differently abundant protein spots by spot intensity quantification using the PDQuest software, we found a total of 21 over-expressed proteins in patients than in control subjects (considering an expression difference >1.5-fold).
In our study, we used the same substrate (LPP) as [McDonnell et al. (1997]), but a different detection method because quantification using the ninhydrin detection method was inappropriate in our case (data not shown).
One plate of each condition was used for protein quantification using the BCA protein assay kit (Pierce, Rockford, IL, USA) according to the manufacturer's recommendations, and bovine serum albumin was used as the protein standard.
Hence, the quantification using the more internal peptide (209 220) was more relevant.
Bands were selected for quantification using the automated volume contour tool.
Intensity of immunoreactive bands was estimated by densitometric quantification using the Gel analyzer v1.0 software.
For both types of gene expression the target genes were quantified by relative quantification using the comparative CT method.
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