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No other quantification used blinding.
Previous studies investigating the accuracy of 177Lu quantification used a number of different phantom configurations, and different data acquisition and processing methods.
2) Library release from beads before quantification used the heat-release protocol advocated by Maricic and Pääbo [28].
To ensure that quantification used an exposure that was within the linear range of the film used to image the blot, a series of images with increasing exposure time to the blot were developed.
The method of viral load quantification used in the study has been certified by the Virology Quality Assurance at Rush University, Chicago, IL, as a part of the laboratory proficiency testing.
All immunological quantification used proteins transferred electrophoretically to Hybond-P membranes (Amersham Biosciences), incubated with the radiolabeled secondary antibody, exposed for 1 3 days at room temperature with a Phosphor screen, and the screen imaged using a PhosphorImager scanner (Typhoon 9200, Amersham Bioscience).
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An aliquot was kept for protein quantification using the Lowry method.
Drug combination studies and their synergy quantification using the Chou-Talalay method.
Klein, D. Quantification using real-time PCR technology: applications and limitations.
In BAC-Exp5 animals, expression levels were too low for reliable quantification using a single round of RT-PCR.
Garber, M., Grabherr, M. G., Guttman, M. & Trapnell, C. Computational methods for transcriptome annotation and quantification using rna-seq.
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