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Because luciferase reporter can provide a cost-effective way of quantification, together this dual reporter system offers a powerful tool superior to any single reporter system.
This precise quantification together with evidence from CaptureSeq that the sequenced fragments are samples from complete transcripts (and not just 'noise') points towards the presence of very rare transcripts, possibly even unique to individual cells.
In addition to supplementing lung tissue material with other non-solid organs like blood, urine, and BAL, also the combination of multiple methods like omics, immunohistochemistry, protein assays, and mRNA quantification together with clinically meaningful endpoints may well prove to be the most beneficial approach with which to discover relevant non-invasive biomarkers.
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There is currently a lack of a reliable resource for end users to learn about these tissues' imaging and quantification techniques together.
In plants, like in other organisms, the improvement of gene expression quantification technologies, together with growing databases, supports this development considerably.
One of the papers selected for this special issue reviews the turnover rate of phenolics and its quantification in soils, together with their regulatory effects on decomposition.
A typical western blot together with quantification of the PrxV bands is shown in Fig. 2I.
The total CSFV genomic RNA copy number was determined by real-time RT-PCR [ 27, 31] together with quantification standards.
The HPLC finger print of the chloroform extract together with quantification of Indirubin as marker is also given.
Taken together, the quantification of CEC by CD146 real-time PCR showed equivalent results to the flow cytometry analysis.
The recovery and quantification of genomic DNA together with RNA has previously enabled us to establish genome number averages for developing embryos [ 17, 19].
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