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The most intense pair (72/55) was used for quantification, the two less intense transition pairs were used as qualifiers for further compound confirmation.
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For quantification, the three dimensional volume around each cellular location was extracted from the full z-stack and intensity was integrated over a consistent number of the brightest pixels, which fully encapsulate the entire cell in all cases.
For microvessel quantification, the three most highly vascularised areas detected by CD34 immunostaining were initially selected under the × 40 field, and a × 200 field (0.785 mm per field) was used to count vessels in each of these areas.
For microvessel quantification, the three most vascularised areas detected by CD34 immunostaining were initially selected under × 40 field, and × 200 field (0.785 mm per field), and microvessels were counted in each of these areas.
The present work describes a novel method tracer studies with a mixture of native and fluorescent-dyed bacteriophages that facilitates parallel quantification of the two processes.
Since lattice strain and charge density affect various material properties of graphene, a reliable and efficient method is required for quantification of the two variables.
The scenario quantification for the two scenarios (a) "income first" and (b) "ecological benefits" covers a time period of 20 years.
While the quantification of the two biological outcomes depends on specific model and implementation details, the main message is clearly encapsulated in the network: PI3K inhibition does not eliminate all the proliferation-inducing, apoptosis-resisting activity in the network.
Therefore, the differential detection and quantification of the two isoforms is of critical importance for the design of a more specific inhibitor that targets only the elevated isoform.
Figure 3 shows the concept for the quantification of the two different phosphorus candidate CRM.
Quantification of the two DON-sulfate isomers was done by matrix-matched calibration curves (1/ x weighted).
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