Sentence examples for quantification the samples from inspiring English sources

Before the protein quantification, the samples were diluted in the appropriate buffer so that the concentrations determined would fall within the assay and equipment limits.

For quantification, the samples were normalized against the expression of GAPDH, TUBA1C, HSP90AA1 and 18S mRNA.

After RNA quantification, the samples were pooled so that an equal amount of each was present and send on dry ice for further processing to GATC-Biotech (Germany).

After protein quantification, the samples were used to determine MDA level by method of Ohkawa et al. [ 42] and Txnip, ICAM-1, and p47phox expressions by Western blot analysis [ 6, 7, 21, 24].

For the pre and postsynaptic marker quantification the samples were double labeled with anti-HRP and the marker of interest: the mean intensity of both the marker and the HRP was quantified and a ratio calculated, adapted from (31).

Following cleanup and quantification, the sample was titrated to optimize yield and sequence quality.

Quantification of the samples was performed according to the threshold cycle using the ΔΔCt method.

The use of bisphenol F as internal standard allowed quantification of the samples against aqueous standards.

The software was coded in Matlab for segmentation, morphological operation and color quantification of the samples.

Artificial neural networks (ANN) were used for color quantification of the samples.

The use of d10-phenanthrene as internal standard not only improved the repeatability of the method but allowed quantification of the samples against external aqueous standards.