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To provide quantification, the point of product accumulation in the early logarithmic phase of the amplification plot was defined by assigning a fluorescence threshold above the background, defined as the threshold cycle (Ct) number.
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For quantification the crossing point method was employed.
However, if sufficiently large volume of interest was used, accuracies of quantification obtained from the point source and water cylinder were similar.
For filopodia quantification the first time-point of a 2-min video was analysed and filopodia dynamics was followed over a time-period of 10 min. For filopodia measurements the manual mode of the 'filament tracer' module of the Imaris software (Bitplane, Switzerland) was used.
For this mutant, the conclusion is that even though the mutation impacted on the quantification of join points, the behaviour of the woven application remained the same.
Calibration curves for the antioxidants catechin and EGCG were generated from fresh solutions and used for the quantification where each point in the calibration curve is the average of triplicate injection.
In effect, this point is just a more-formal version of the point about quantification that was made back in section 2.2.
The quantification of end point RNA indicated the presence of viable cells, an observation supported for all tested parasites by their growth recovery 7 10 days after drug withdrawal (Figure S1).
As no SCX fractionation was performed before the Target-SRM/MS2 analysis, a longer ACN gradient (from 3 to 30% in 140 min) was applied to obtain an optimal number of data points along the peak elution profile for the quantification (DATA points >10).
From the point of view of quantification, clustering algorithms may be useful for the detection of weaker peptides.
Current research issues include the quantification of non-point sources, the atmosphere biosphere exchange of ammonia, the quantification of landscape processes and the primary and secondary emissions of PM.
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