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In contrast, some of the details including the lower limit of quantification, the linear range of quantification, the accuracy, and the relative standard deviation of the method used in the 2012 study are not available.
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The limit of quantification and the linear range of calibration curve were 4.6 μg mL− 1 and 0.1 25 μg mL− 1, respectively.
The proposed biosensor was very sensitive (22.4 μA cm−2 mM−1) in glucose quantification, and the linear range was from 1 μmol L−1 to 2 mmol L−1 with the detection limit of 0.8 μmol L−1.
The number of cycles for amplification was determined empirically to allow quantification in the linear range of PCR.
The PCR reaction was stopped after a total of 40 45 cycles and at the end of each extension phase, fluorescence was observed and used for quantification within the linear range of amplification.
Correlation is a quantification of the linear relationship between variables and does not examine the question of agreement.
To estimate the accuracy of the quantification method, the linear response of AMPs to the internal standard BSA (which was assessed for linear responses within the used concentrations) was determined by analyzing serially diluted samples.
These new data, including quantification in the linear range, are included in Figure 4F-G and referred to in the revised text in the subsection "Ciliary targeting of D1Rs is promoted by IFT-B comproteinsteins and KIF17".
Quantification of the linear-relationship between the various parameters measured in vivo and ex vivo were analysed by Pearson's correlation to provide the correlation coefficient (r) and the significance (p).
The lower limit of quantification was 200 pg/mL and the linear quantification range up to 5,000 pg/mL in ascites as biological matrix.
A high background signal for blank control samples containing no analyte worsens the limits of detection and quantification for real samples and limits the linear quantification range of an assay.
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