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After quantification, the density of staining was calculated by dividing the pixel count by the cell number to get pixels per cell.
For quantification, the density of pixels of each band was determined by the NIH Image J 1.45s software (available at http://rsb.info.nih.gov/nih-image; developed by Wayne Rasband, NIH, Bethesda, MD).
For quantification, the density of bands from three blots was compared using Image J. 12-week-old male Sema3AKD mice and their littermates not expressing Cre (as controls), were used for surgery as above, with five mice for each group and time point.
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For quantification, the densities of bands were determined using Gene Tools software (Syngene), and normalization to the corresponding loading control.
By using high-resolution computed tomography (HRCT), mild emphysema may be assessed by quantification of the density distribution [6].
Quantification of the density of OT-I cells per mm2 of RP and WP confirmed this impression, indicating that a transient clustering of L.m-specific memory CD8+ T cells in the RP precedes their relocation to the WP (Figure 5B).
Quantification of the density of the western bands was done with program ImageJ (http://rsb.info.nih.gov/ij/).
This method of quantification measures the density of immunopositive objects independent of their individual intensities.
Quantification of the density of PNNs showed no significant differences between WT (n = 9) and rl−/− (n = 12) V1 (t test, p = 0.234; Fig. 8g).
Quantification of the density of positive staining for androgen receptor in the intima and media was performed for each vessel utilizing ImagePro Plus (Media Cybernetics).
Quantification of the density of OHCs in Fgfr1 −/f ::Foxg1 Cre/+ embryos demonstrated a significant (p < 0.0001) decrease compared to controls (Fgfr1 +/f ::Foxg1 Cre/+ ), while the density of IHCs was not changed (p > 0.09).
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