Sentence examples for quantification the cell from inspiring English sources

For quantification the cell culture supernatants were diluted 2 fold and the cell extracts 5 fold with calibration buffer.

After protein quantification, the cell extract was resolved by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electroblotted onto nitrocellulose membranes (Bio-Rad).

During quantification, the cell suspension was visually inspected for the presence of clumps using microscopy.

For quantification, the cell index at the indicated time points was averaged from three independent measurements.

For protein quantification the cells were lysed by a CHAPS-based lysis buffer and the protein was estimated by the DC Protein Assay kit from BIO-RAD according to manufacturer's protocol.

For DNA quantification, the cells were fixed in ice cold 80% ethanol and then were incubated for 15 min at 37°C in PBS containing 100 µg/ml DNase-free RNase (Sigma), and 10 µg/ml propidium iodide.

For quantification, the cells were counted under a microscope in 5 predetermined fields at ×200.

For DNA quantification, the cells were transferred to a 96-well plate for the measurement of fluorescence intensity, using excitation and emission wavelengths of 355 and 460 nm, respectively (Spectra Max Gemini, Molecular Devices, USA).