Suggestions(2)
Exact(5)
For correct quantification, the amount of cDNA used for the amplification of GAPDH was adjusted according to the amount of each of the amplified genes.
After the incubation period, the cells were washed and whole cell lysates for comparative quantification (the amount of proteins in unstimulated cells were set to 1) of total protein concentration prepared.
For quantification, the amount of miR was normalized to the amount of U6 miR using the 2 − DDCT method.
For a reliable mass-spectrometric quantification, the amount of tissue obtained from our experimental setup is limiting.
Based on band quantification, the amount of Tim50 extracted by SMA at the concentration used was nearly equivalent (within 5%) to the amount extracted by DDM.
Similar(54)
When including proteolytic cleavage products in the quantification, the total detected amount found in the cells was around 1200 mg/L.
Additional groups (control and 500 microg/m(3)) were studied by means of morphometric analyses (quantification of the amount of intraepithelial and surface mucins), measurement of cilia beat frequency, and quantification of total glutathione.
Quantification of the amount of free and bound activity was performed in a γ-counter.
Quantification of the amount of free, acid-releasable, and internalized activity was performed in a γ-counter.
Quantification of the amount of free, PMPA-releasable and internalized activity was performed in a γ-counter.
Hence, the method is suitable for quantification of the amount of erythritol crystals present in aqueous solutions and gels, respectively.
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