Sentence examples for quantification target from inspiring English sources
Each quantification target was amplified in triplicate samples and a control lacking the template for each master mix was always included in the experiments.
Each quantification target was amplified in triplicate samples and negative control lacking RT enzyme was always included in the experiments to ensure for any genomic contamination.
For more precise quantification, targeted PCR reactions were carried out, and the amplified products were analyzed by automated chip-based microcapillary electrophoresis on an Agilent 2100 Bioanalyzer instrument.
For more precise quantification, targeted PCR reactions were carried out, and the amplified products were analyzed by automated chip-based microcapillary electrophoresis on an Agilent 2100 Bioanalyzer instrument (Agilent Technologies, Santa Clara, CA) as previously described [ 37].
The development of quantitative proteomics has enabled the absolute quantification of target proteins (i.e., target proteomics).
Recent advances in bottom-up proteomics using targeted LC-MS have enabled previously unachievable identification and quantification of target proteins and posttranslational modifications within complex samples.
Use of internal reference gene(s) is necessary for adequate quantification of target gene expression by RT-PCR.
In this study, a new method for the absolute quantification of target proteins was developed by combining dimethyl labeling with SID-MRM mass spectrometry.
The absolute quantification of target proteins in proteomics involves stable isotope dilution coupled with multiple reactions monitoring mass spectrometry (SID-MRM-MS).
Furthermore, chemical ionization quadrupole mass spectrometer which is available switching between EI and CI techniques was suited better than EI-fast TOFMS for the quantification of target alkylpyrazines.
Three replicates were performed for each reaction, and OsActin1 was used as an internal control for the relative quantification of target gene expression.
