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The high reproducibility in expression levels avoids protein purification and quantification steps for false positive variants and avoids real positives from being discarded.
The reproducibility of the quantification results obtained from the different samples demonstrates the robustness of the extraction and quantification steps (Table 3).
The next-generation phage display approach introduced here includes DNA-analysis of clones from the initial quantification steps to the final large-scale sequencing, which are relatively much faster and far less expensive.
Subsequent detection and quantification steps were as described above.
All subsequent quantification steps were performed according to the manufacturer's instructions.
Reverse transcription to generate cDNA for use in quantification steps was conducted as described in Subramanyam et al. [ 57].
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Precise measurement of the total volume of supernatant and washing solutions recovered is very important in the quantification step.
The additional quantification step aims at removing duplicate symbol vector outputs in the case of a list of solutions.
This point is transparent with classical detectors such as LD and DFD, thanks to the straightforward quantification step in the reduced domain [39].
After extraction, DNA was quantified with a Nanodrop 2000 (ThermoScientific) [Note: for large classes, the quantification step could be skipped, all extractions contained DNA (from two ηg/μl to 20 ηg/μl) and all PCRs were successful].
The application of robust model-based design concepts for complex chemical processes is limited due to the repeated cpu-intensive uncertainty quantification step for any new tested process design configuration.
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