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Amplicon carrying plasmids were used in concentrations of 105 and 102 copies/μl as quantification standards.
Additionally, the standard recovery rates of 13C12-labelled quantification standards were checked.
In particular, each centre adhered to their manufacturer's recommended quality control schedule, including quantification standards at minimum six monthly intervals.
The standard recovery rates of 13C12-labelled quantification standards were checked to be in the range of 50% to 150%.
Additionally, the standard recovery rates of 13C12-labelled quantification standards were checked to be in the range of 50% to 120%.
The lack of DNA RMs and acknowledged DNA quantification standards, hinders the result mutual accredit and explains the significance of the consistency, uncertainty and traceability of DNA analysis methods.
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For isotopic quantification, standard lactose was dissolved in deionized water by step-wise dilutions to obtain the concentrations ranging from 50 to 800 ng μL−1.
However, 2-naphthol (quantification standard) and deuterated phenol (D6-phenol as a measure for loss of volatile phenol) standards were added to the substrates prior to separation by C18 non-endcapped SPE.
The assay method was absolute quantification (standard curve).
Data under the detection threshold (outside of the quantification standard range) were excluded from the analysis.
This quantification standard reports the percentage of units containing at least one fusion event.
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