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Traditional posturography analysis and 4 nonlinear time-series analyses (sway density plots, diffusion plots, recurrence quantification, sample entropy analyses) were applied to the recorded CoP time series, based on which 13 traditional and 32 nonlinear time-series CoP-based measures were calculated.
Homogenization of cardiac tissue, protein quantification, sample preparation (40 μg per lane) and electrophoresis were performed as previously described [ 14].
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Consequently, for accurate quantification, samples need to contain an activity lower than 100 kBq.
Prior to quantification, samples were washed with 10%% trichloroacetic acid during 30 min at 4 °C.
For protein quantification, samples of unknown GFP concentration were loaded in triplicate along with the GFP standards of known concentration.
For GSSG quantification, samples were pre-treated with 5% (v/v) 2-vinylpyridine for 1 hour at room temperature before analysis.
Within each ELISA quantification, samples were run in triplicate.
After DNA quantification, samples were adjusted to TE buffer, partitioned into aliquots, and stored at -80°C.
For sugars and metabolites quantification, samples were harvested at stationary phase and analyzed using HPLC [ 25].
After DNA quantification, samples were adjusted to TE (Tris-EDTA) buffer, partitioned into aliquots, and stored at −80°C.
For quantification, samples included a known quantity of beads (TruCOUNT™, BD Biosciences, San Jose, CA, USA) of 4.2 μm diameter.
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