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An analysis of the uncertainty quantification related to LBLOCA using the Monte-Carlo calculation has been performed and compared with the tolerance level determined by the Wilks' formula.
This method addresses an important source of uncertainty for dPCR-based quantification related to whether the template DNA is in single-stranded or double-stranded form [ 38].
The criteria for detecting the most likely K was the Δ K, an ad hoc quantification related to the change of a second order in the logarithmic probability of the data in relation to the number of groups inferred by the Structure algorithm [ 32].
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Relative quantification relates the PCR signal of the target transcript (here FA or HA phenotypes) in a treatment group to that of another sample (here C phenotype).
For the analysis of GmMADS28 expression in the soybean mutant, the relative quantification relates the PCR signal of the target transcript in the organs of the mutant to that of the wild-type plant.
To verify the microarray data for GmMADS28 expression, the relative quantification relates the PCR signal of the target transcript in the roots, flower mixtures, or pods to that in the leaves.
The techniques proved reproducible for both nMVD and aMVD quantifications related to total tumour area.
Based on these data, the concept for an appropriate quantification and related spatial visualization of ecosystem service supply and demand is elaborated and discussed.
Due to the difficulties and expenses to prepare all the related reference standards, the application of the external standard method was limited, so correction factor was used in the quantification of related substances.
The percentage recoveries are tabulated in Table 2. Standard addition and recovery experiments were also conducted to determine the accuracy of the present method for the quantification of related compounds A, B, C and D. The range of addition levels of impurities to the parent compound was done at 0.15-0.75% of the concentration (10 μg/ml) of the FEX [18].
For quantification, GagProPol related bands on the film were scanned and densitometry performed using Un-Scan-It (Silk Scientific Corporation).
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