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A dynamic quantification range from 0.125 ng mL−1 to 16 ng mL−1 was obtained with both types of aptamer sequences while the detection limits were 0.12 ng mL−1 and 0.25 ng mL−1 for seqA and seqB respectively.
When GE-maize cultivars MON81 and NK603 were tested with real-time PCR [ 54], a quantification range from 0.5%to100%0% in 100 ng of non-GE DNA was reported.
In brief, the CAP/CTM CMV test uses primers and probes targeting a conserved region of the CMV genome (UL54, virus encoded DNA polymerase) and has a linear quantification range from 150 to 10 000 000 (2.18–7.0 log10) copies/mL representing 137 and 9 100 000 (2.14–6.96 log10) IU/mL, respectively (1 copy = 0.91 IU).
This PCR assay targets the CMV UL54 gene (DNA polymerase) and displays a limit of detection of 100 copies/ml (91 IU/ml), a limit of quantification of 150 copies/ml (164 IU/ml), and a linear quantification range from 150 to 10,000,000 (2.18 to 7.0 log10) copies/ml for plasma specimens.
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Limit of quantification ranged from 0.02 to 0.08 mg kg−1.
The limit of quantification ranged from 4.48 to 11.7 μg/L.
The limit of quantification ranged from 0.2 μg/kg to 5 μg/kg.
The limit of quantification ranged from 0.1 to 10 ng mL−1, and the recoveries were 34.6 50.3%.
The limits of quantification ranged from 1.0 μg/kg to 12.5 μg/kg for the dinotefuran and UF enantiomers.
The limit of quantification ranged from 0.063 pg m−3 to 0.210 pg m−3 when air volumes of 723 m3 were collected.
Under optimized conditions, the limits of detection and quantification ranged from 0.04 to 0.09 and 0.15 0.29 μg L− 1, respectively.
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