Nevertheless, some differences obtained were statistically significant, namely, the quantification of probe in the blood, sternum, heart, spleen, kidney, and muscle.
Quantification of probe signal was preformed using NIH Image software.
The GenePix Pro 4.0 software was used for identification and quantification of probe intensities.
This three-color approach allows for assessment of slide fabrication independent of hybridization, thereby enabling 1) direct visualization of array/element morphology, 2) quantification of probe deposition and retention on the slide surface and 3) ultimately a means for array quality control prior to hybridization.
On the basis of these results, an adsorption time of 30 min was considered as a suitable value to obtain an adequate analytical sensitivity at a reasonable analytical time and it was applied for all the next SPME quantification of probe compounds.
In this context, the precise quantification of probes in tissues is a matter of concern in biodistribution studies.
Quantification of probe-dependent cooperativity factors can provide a mechanistic framework within which to understand the influence of dimerization on ligand-GPCR interactions and, as such, develop structure-activity relationships for ligands interacting across a dimer interface.
The SPME analytical procedure applied permitted a fast and reliable quantification of the probe compounds without affecting the interactions probe compounds/nanomaterials.
The experimental design was aimed to the identification and quantification of the probe compounds adsorbed by the NPs with the optimization of the analytical parameters and the amount of probe compounds and NPs required for the adsorption test.
The study objective was to assess the potential of high performance liquid chromatography - inductively coupled plasma - mass spectrometry (HPLC-ICP-MS) for quantification of molecular probe metabolism and pharmacokinetics using stable isotopes.
