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Exact(5)
The Ki67 staining results mirrored those obtained with PCNA, and served as a more accurate marker to use for the quantification presented in the side graph (Fig. 4A,B).
The result of this experiment is shown in Fig. 4a, with the relative quantification presented in Fig. 4b.
However, we want to emphasize that the quantification presented in the first manuscript was not exclusively of the image shown, but from all three experiments performed.
The quantification presented in Figure 6B shows that percentage of Fucci-green (i.e. S and G2 cells) progressively dropped and is decreased by a two-fold factor after 6 days.
For each gene, each phenotype reported was observed in at least two independent experiments, and observed for at least 70% of embryos examined, using MO amounts that had been titrated (Table 2) and gave a clear phenotype (with quantification presented in supplementary material Table S3).
Similar(55)
Because of the significance of the effect of hydrodynamic interaction on the number of active nucleation sites and bubble size at detachment, mechanistic modeling of nucleate flow boiling is expected to benefit from the quantifications presented in this study.
A notable example are the single molecule quantifications presented in Figures 8 and 10, which demonstrate that real-time qPCR can be a remarkably accurate technology.
Protein quantifications presented in this report were normalised with respect to β-actin or GAPDH.
The ultimate objective, however, was to assess the absolute quantitative accuracies by comparison to the LDA quantifications presented in Table 3.
The scenario quantification methodology presented in this contribution reflects the BEAST approach [29, 44, 100] and could be used as a blueprint for other regions independent of the goals or the spatial configuration.
The quantification was presented in the Figure 4c.
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