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Analytical performance was evaluated in terms of specificity, linearity, limit of quantification, precision, accuracy, recovery and repeatability.
For validation a method-performance study was conducted involving the establishment of the analytical performance of the new method (limits of detection and quantification, precision and accuracy).
Validation was conducted according to the method-performance study to estimate the performance characteristics of the new method (limit of detection, limit of quantification, precision and accuracy [50, 51].
Dilutions of RT products are often used to minimize post-RT inhibitory effects [5], [10], [12], [13], [17], [18], although this has the negative consequence of reducing quantification precision, which is especially problematic when dealing with very low copy templates, such as those obtained from archival tissues.
In discussion we outline possible experimental and computational strategies for further improvements in quantification precision.
Quantification precision (CV%: 10 22%) was sufficient here to allow semi-quantitative estimation in the low fentomole range.
Similar(50)
The quantification precisions in the five datasets were also measured with RSD of bootstrap estimates.
The method was validated in terms of limit of quantification, accuracy, precision and linearity.
The SPME-GC-MS/MS method was validated in terms of linearity, accuracy, limits of detection and quantification, and precision.
It was based on the following criteria: limit of detection (LOD), limit of quantification (LO Q, precision (intra- and interday variability), recovery, and linearity.
In this case, the following issues have to be addressed: detection and quantification limit, precision, stability of analyte, specificity, interfering compounds etc.
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CEO of Professional Science Editing for Scientists @ prosciediting.com