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The relatively small variability of the nebulizer output (5%%) [6] and images quantification performed by two blind clinicians ensured the accuracy and the reproducibility of the analysis.
Radioactivity quantification in regions of interest drawn on tumors and normal tissues can be performed on planar scintigraphy (2D) but organs superposition limits its capacity to precisely approach AD. 3D quantification performed on SPECT/CT images has been proposed with improved results when compared to 2D, e.g., [5, 6] (among others), but is not yet widely available.
Through Lyve-1 immunolabeling on tumor sections, quantification performed by computerized image analysis led to the determination of 3 different parameters (Fig. 2B, C, D).
The slides were examined with a fluorescence microscope (Olympus BX61) and quantification performed using the Image Pro-Plus 5.0 software (Media Cybernetics, Bethesda MD, USA).
Developed films were imaged on a Bio-Rad GS-700 Imaging Densitometer, and quantification performed on background-subtracted bands with Molecular Analyst (v. 21) Software.
Amino acids and biogenic amines were quantitatively analyzed by reversed phase LC-MS/MS to obtain the chromatographic separation of isobaric (same MRM ion pairs) metabolites for individual quantification performed by external calibration and by use of internal standards.
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Protein quantification was performed using intensity-based quantification (iBAQ).
Image analysis and quantification were performed using Inveon Research Workplace 4.2 (Siemens Medical Solutions).
Quantification was performed by the standard curve method.
The quantification was performed using the internal standard method.
Quantification was performed using a five-point calibration.
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