YB assisted in the quantification of the specimens.
These data thus demonstrate the application of the real-time RT-PCR assay for the detection and quantification of PIV3 in clinical specimens.
This novel approach provides a useful tool for the quantification of biomarkers on tissue specimens, as well as for objective identification of appropriate cutoff thresholds for biomarker positivity.
Quantification of FCT in the specimens was permitted by the morphometric software LUCIA Net version 1.16.2© (Laboratory Imaging, Praha, Czech Republic), Laboratory Imaging using NIS Elements 3.0® (Laboratory Imaging).
Should more than one respiratory viral or bacterial pathogen simultaneously appear in respiratory specimens, quantification of individual nucleic acids may help identify the dominant agent within these classes of pathogens by comparing cycle threshold (Ct) values [ 31].
Beggs et al. [ 35] used a methylation analysis based on bisulphite conversion and whole-genome amplification and found that in ten colorectal cancer and five colorectal adenoma specimens, quantification of whole-genome methylation did not correlate with previously published CIMP criterion.
Twenty-five women and 15 men completed a questionnaire, and provided biologic specimens for quantification of Hg, Pb, Cd, and As using ICP-MS.
These assays will be useful in studies to determine how persistent alphavirus infections are maintained in the vector host, and in the detection and quantification of replicating virus from clinical specimens and potential reservoir hosts.
Digital PCR has been applied in quantification of EGFR mutants in clinical specimens, providing a promising molecular diagnostic tool [ 28].
Generally, real-time RT-PCR was used for the detection of ANXA1 mRNA expression, while Western blotting and immunohistochemistry were established to visualize the tissue-specific expression pattern and quantification of ANXA1 protein in these specimens.
