The DNA tail was engineered to include a single ScrFI restriction site, which enabled subsequent quantification of restricted DNA tails using real-time PCR.
The 'library' of drought information from this study, paralleled by sensitivity analysis, will be useful during drought for reservoir scenario projection and quantification of the risk of restrictions to supply, such as the effect of a ban on the use of hosepipes; in 'planning mode' for testing the robustness of Drought Plans; and for application to other catchments across the region.
To implement this idea, we improved adaptor-tagged competitive PCR, originally developed for relative quantification of the 3'-end restriction fragment of each cDNA, such that it can be applied to any restriction fragment.
Telomere length studies were started around 1980s and were initially based on Southern blot quantification of a smear of telomere restriction fragments, the so-called terminal restriction fragment (TRF) analysis [ 22- 24].
Assessment is particularly valuable in patients with LSS, to allow objective quantification of the actual extent of restrictions and to address this topic with the patient.
A pooled quantification of the inhibitory effects of caloric restriction on spontaneous mammary tumour incidence in mice showed that caloric restriction lowered the incidence with 55% [ 6].
Improvements in technology will permit quantification of the absolute rate (incidence) of growth restriction at each gestation in the future.
The copy number of clade II was determined using polymerase chain reaction (PCR) amplification followed by restriction enzyme digestion and quantification of the two digested products.
This is followed by restriction enzyme digestion and quantification of the two peaks produces a ratio of cut to uncut products.
In this study, we investigate the suitability of a range of methods based on bisulfite conversion and restriction enzyme digestion for accurate quantification of methylated DNA copies.
Quantification of exon IIIb and IIIc for Fgfr1, Fgfr2, and Fgfr3 required restriction enzyme specific to discriminate the two isoforms.
