The authors also discuss the potential of PCR methods for quantification of salmonella in pig faeces.
(b) Quantification of PIP2 levels.
The aim of this study was to evaluate the use of flotation, a non-destructive sample preparation method based on traditional buoyant density centrifugation, for culture-independent quantification of intact Salmonella in pig carcass gauze swabs (100 cm2) prior to quantitative PCR (qPCR).
Computed tomography has proven equal to manual dissection for quantification of lean tissue in pigs [ 19– 21], has practical advantages over MRI [ 22, 23] and is therefore used to select boars with optimal body composition in some countries [ 24, 25].
Furthermore, hemagglutination assay demonstrated an excellent positive correlation with bioadhesion quantification in human endothelial cells and the endothelial layer of pig aorta thus validating the hemagglutination assay described here as a useful method for predicting nanoparticle bioadhesivity to vascular endothelium.
This investigation offers the first experimental data on the JARs and BSPs of pigs' limbs, thus enabling a quantification of net joint forces and moments.
The new CT scoring system allows for quantification of gross pathological lung alterations in living pigs.
Hammad, S. et al. Protocols for staining of bile canalicular and sinusoidal networks of human, mouse and pig livers, three-dimensional reconstruction and quantification of tissue microarchitecture by image processing and analysis.
The established qPCR is sensitive, specific, and reliable, which could be a useful tool when applied to quantification of P1 in a variety of samples from infected pigs.
What was lacking, however, was the quantification of 'home range' size and of habitat preferences of the pigs within this free range system, important elements of an understanding of the disease risks to which free range pigs are exposed.
