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Therefore, this method was used for quantification of neurons (Figure 1E).
Quantification of neurons, i.e. particles, is important for revealing degrees of neurodegeneration and regeneration in an organ or system.
Quantification of neurons was performed according to the principles of unbiased stereology [ 31].
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As neurons are very difficult to grow, neurobiologists heavily depended on the established research methods, and Dr. Tchou's work on the quantification of neuron cells and rhythmic discharge in Aplysia, as well as the experimental methods established during the researches, laid a solid foundation for the studies using Aplysia as a model organism.
The quantification of neuron survival and neuropathological lesions in neurodegenerating tissues is essential for the development of therapeutic strategies relying on the delivery of trophic factors [ 26].
It is interesting to compare the quantifications of neurons carried out by various techniques.
(C ) Quantification of neuronal proportions TH+ neurons that are IB4−SNS-Cre/TdT+, IB4−SNS-Cre/TdT+, or Parv-Cre/TdT+ neurons expressing TH.
Quantification of motor neurons at 30 hpf showed that the number of islet-1-positive spinal motor neurons was significantly lower than in controls (Fig. 3I).
Blinded quantification of intact neurons in the striatum and motor cortex after cresyl violet staining revealed a preservation of neuronal number after DMF treatment (Fig. 7C,D).
Rosenthal's canal served as the reference space in these experiments and design-based stereology was employed in first-order stereology quantification of immunoreactive neurons.
(A) Schematic diagram of the experiment process; (B) TH-immunoreactive cell bodies in SN of different groups of rats; (C) Quantification of DA neurons loss among different groups of rats, *P < 0.05 (n = 10 in each group); (D) The Kaplan-Meier curves demonstrate the probability of disease onset, *P < 0.05.
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