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(D) Bar graph quantification of mean fluorescent intensity analyzed using the FlowJo software v7.6.1.
The poor spatial resolution also affects the quantification of mean absorbed dose due to the spill-out effect, which is resolution-induced mispositioning of activity in the image and causes underestimation of the activity concentration in volumes with high activity.
Superoxide production was quantified by two techniques: 1) by adding hydroxylamine (0.5 mM) [12] during cell culture, which converts superoxide into nitrite plus nitrate, and which was reduced by VCl3 [13] and quantified using Griess reagent; and 2) by hydroethidine (Invitrogen) staining and quantification of mean fluorescence intensity (MFI) cells by flow cytometry (FACSort; BD Biosciences) [11].
(A) Quantification of mean diameter of moving vs stationary axonal BRP punctae.
Depicted values represent quantification of mean grey values of corresponding bands.
Quantification of mean intensity over background for each animal was performed using Fiji (http://fiji.sc).sc
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Quantifications of mean signal intensities for each spot were determined using the GenePix Pro 5.0 software (Axon Instruments, Inc .. Normalization and t-statistics were carried out using the EMMA 2.6 microarray data analysis software developed at the Bioinformatics Resource Facility Center for Biotechnology, Bielefeld University http://www.genetik.uni-bielefeld.de/EMMA/[ 54].
The quantification of the mean background intensity and mean fluorescence intensity of Spc97-GFP and Spc110-GFP signals at SPBs was performed on planes having SPBs in focus.
(F ) Quantification of the mean intensity of extracellular Dpp-GFP signals that colocalize with integrin/Mys signals in control or vkg mutant guts (mean ± SD: n = 15 for each genotype).
(F) Bar graph quantification of the mean fluorescent intensity analyzed using the FlowJo software v7.6.1.
(D) Quantification of the mean vessel density demonstrates a significantly higher vessel density in the control group, **P < 0.001.
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