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Direct quantification of lymphocytes present in the biopsy used for RNA extraction has not been possible.
Due to limited sample size not all laboratory investigations regarding quantification of lymphocytes, RTL or TRECs could be performed in all patients.
Quantification of lymphocytes from adipose tissue demonstrated reduced numbers of CD4+ T cells, CD8+ T cells, and NK cells, and a reduced production of inflammatory cytokines, including IFNγ, TNFα, IL-1β, and IL-6.
Quantification of lymphocytes subpopulations was performed by using BD Trucount tubes (BD Biosciences, San Jose, CA, USA) for enumeration of mature human T (CD3+), B (CD19+), helper/inducer T (CD3+CD4+), suppressor/cytotoxic T (CD3+CD8+) and NK (CD3-CD16+CD56+) lymphocytes by using a BD FACSCalibur 4-color flow cytometer (342975; BD Biosciences).
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Therefore, pharmacokinetic modelling might be required for accurate quantification of lymphocyte infiltration.
Quantification of lymphocyte subsets and evaluation of their function could thus have diagnostic and prognostic value in sepsis.
Thus, we have a 100% correlation between the histopathological examination and the qRT-PCR quantification of lymphocyte-specific mRNAs.
Flow cytometry quantification of lymphocyte subsets, and lymphocyte response to mitogens were performed at Day +30, +60, +100, and +180, and TREC and Vβ spectratyping were performed at Day +100 in all patients who were alive without relapse.
Linear regression analysis revealed no significant correlation between the individual PASI-scores and the quantifications of lymphocyte subsets or cells with NK-cell receptors.
Figure 5 Quantification of T lymphocytes.
All parameters tested, which included standard histological examination, measurement of epidermal thickness as well as detection and quantification of CD3+ lymphocytes, mast cells, and eosinophils, yielded no evidence of consequences of CMS application when compared to skin treated with vehicle alone (Fig. 3).
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