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Quantification of label is determined from the total integral optical density of labeling.
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Right panel shows the quantification of labeled mtRNAs (n = 3).
Next to cell visualization, quantification of labeled cells implanted in cartilage defects by MRI could provide an exquisite opportunity to longitudinally map the role and contribution of implanted cells to cartilage repair.
Because EM analysis of sectioned cells does not allow an accurate quantification of labeled cells in a population of bacteria, we analyzed the induction of a similar pBAD plasmid allowing the expression of a Hfq-venus fluorescent protein (YFP) in whole cells (instead of MT-tag).
(G and H ) Quantification of labeling density (LD) of albumin and VSVG by immuno-EM, normalized to ER labeling.
Quantification of labeling intensity was normalized to labeling at the 4-hr time point after subtraction of background.
Notably, quantification of labelling patterns of xylulose-5-P, dihydroxyacetone and glyceraldeyde phosphate, which are directly linked to methanol metabolism, could be measured by first time.
For comparative quantification of labelling, the proteins were excised from the SDS gel and the incorporated γ-P was measured by liquid scintillation counting.
Quantification of labeled adiponectin uptake into tissues demonstrated uptake primarily into liver and kidney with no significant differences detected between vehicle and isosorbide dinitrate treatment for HMW adiponectin.
Many of the issues pertinent to the quantification of labeled peptides also apply when analyzing label-free data; however, there exist additional challenges related to comparing peptide abundances across different LC MS data files.
After quantification of labeled DNA, the labeled DNA was hybridized for 72 hr at 42° on the 700K feature or the 1.4M feature NimbleGen aCGH arrays designed for G. max.
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