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As in our previous report, the quantification of iron uptake by tissue macrophages was based on the flow cytometry analysis of cells stained with anti-dextran antibodies.
Details regarding the quantification of iron status indicators are described elsewhere [ 38, 39, 42, 43].
The Fe isotope was used for the quantification of iron content in samples.
C-Iron is a stand-alone software tool dedicated to the voxelwise measurement of T2* decay for the quantification of iron overload and liver PDFF.
For initial cell labelling assays and quantification of iron, the ST14A rat neural progenitor cell (NPC) line was used, and cultured as previously described (generous gift from E. Cattaneo).
A comprehensive method for the quantification of iron saturation in lactoferrin preparations was developed to obtain a calibration curve enabling the determination of iron saturation levels relying exclusively on the defined ratio of absorbances at 280 and 466 nm (A280/466).
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The histological quantification of hepatic iron was carried out according to Deugnier et al (1992) by scoring iron separately within hepatocytes (hepatic iron score, 0 36), sinusoidal cells (sinusoidal iron score, 0 12), and portal tracts or fibrotic tissue (portal iron score, 0 12) using liver samples stained with Perls' Prussian blue.
MRI has been proposed for non-invasive detection and quantification of liver iron content, but has not been validated as a reproducible and sensitive method, especially in patients with mild iron overload.
Figure 3 Prussian blue staining (A) and ICP-OES quantification of intracellular iron contents (B) of RAW267.4 cell incubated with SHU555A or MNCs with average size of 63 nm at different iron concentrations for 1 h.
Quantification of adsorbed iron species was done by atomic absorption spectroscopy (AAS), getting values of 10.9, 14.9, 13.2, 15.9 and 14.9 wt% from 1 to 5 days respectively.
Figure 4 Prussian blue staining (A) and ICP-OES quantification of intracellular iron contents (B) of RAW267.4 cell incubated with SHU555A or MNCs with average size of 63 nm at the concentration of 10 μg/mL for different periods of time.
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