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Macrophage nuclei fluorescence signals allowed for automatic quantification of host cells in multidimensional images by the Imaris program.
Quantification of host species specific reservoir competence can identify animals most responsible for producing infected ticks and therefore increasing risk for human exposure.
The TaqMan® Ribosomal RNA Control Reagent Applied Biosystemss) designed to detect the 18S ribosomal RNA (rRNA) gene was used as an endogenous extraction control and for quantification of host cells.
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The monitoring and quantification of host-cell infection during cell culture-based influenza A virus production needs to meet several goals: Preferably, it should be sensitive, quantitative and robust enough to handle bioprocess modifications such as differences in multiplicity of infection (moi) or cell concentration at time of infection.
Here we present methods for parameter estimation for MDV isolates, allowing a formal quantification of infected host lifespan, viral shedding rates and viral latent periods.
All stereological quantifications for measures of host recovery were analyzed using one-way ANOVA to compare differences between the three groups.
The quantification of bacteria per host cell in various organs was done using the standard curve method (User's Manual, ABI PRISM 7700 Sequence Detection System, Applied Biosystems).
The recent development of techniques for detection and quantification of mycolactone within host tissues has further deepened our understanding of the relationship between this toxic macrolide and clinical disease and has opened up a new vista for utilising mycolactone as a diagnostic and prognostic biomarker for the management of this neglected tropical disease.
The quantification of bacteria per host cell in various organs revealed that of the 14 real-time PCR positive animals, 11 of them had the largest quantities of Brucella DNA in sexual organs (uterus, uterine fluid, testicle or accessory sexual gland), ranging up to 8081 IS 711 copies pro 1000 cells.
The quantification of bacteria per host cell in various organs revealed that in 11 of the real-time PCR positive animals, the largest quantities of Brucella DNA were found in sexual organs, in two animals the spleen was principally affected, whereas in only one animal the largest Brucella DNA quantity was found in urine.
Primer sequences used for qRT-PCR quantification of selected mRNAs from host and pathogen cells.
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