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(B ) Quantification of embryonic mortality at 48 hpf and 72 hpf in WT and hs :ARF embryos exposed to heat shock (left; N = 90, p<0.001).
(A ) Quantification of embryonic mortality at 48 hpf and 72 hpf in wild type (WT and ARFF :ARF embryos (left; N = 90, p>0.05).
This result suggested that by feeding dsRNAs to end-3 (ok1448 ) and wild-type animals in parallel, followed by quantification of embryonic lethality, an RNAi feeding screen could be carried out.
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We describe a procedure that allows the identification and quantification of proteins of embryonic origin, present in attomole concentrations in the blastocoel, the enclosed fluid-filled cavity that forms within 5-day-old human embryos.
For quantification of vascular complexity, embryonic hindbrains are ideal tools to study the potential role of fascin in angiogenic sprouting and vascular remodeling (Fantin et al., 2013).
- Genome-wide quantification of cytosine modifications in embryonic stem cells (ESCs) estimated 5% of all cytosines to be methylated and approximately 0.5% of them to be hydroxymethylated; the higher oxidized forms, 5-fC and 5-caC, are only present in a few thousand to a few hundred copies per ESC.
We show that by direct quantification (Nanostring Assay), that a variety of embryonic globins are expressed that include ζ and ε.
Quantification of Foxd3+ V1 INs in Jag1f/f NesCre embryonic spinal cord at E11.5 revealed that lack of Jag1 function results in a mild, but statistically significant, increase of V1 IN production, when compared to control embryos (Fig. 2 A,C,D).
To examine whether mutants lacking SEMA3s-mediated signaling exhibit defective lymphatic network formation in the embryonic skin, we first established a whole-mount imaging of embryonic limb skin lymphatic vasculature with quantification measurements (Fig. 3A).
(B ) Quantification of numbers of RFP+ cells cultured with no, embryonic PA2, or embryonic heart feeders.
(C ) Graphs show the quantification of second division monopolar spindles (left) and embryonic viability (right) after sas-4 RNAi) sas-4 RNAi with either andT orescue sas-4::gfp transgene.
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